Composite

Part:BBa_K3924044:Design

Designed by: Wenda Zhang   Group: iGEM21_Tsinghua   (2021-10-21)


mchI-mchB-mchX polycistrons with Ptac lacO promoter


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 99
    Illegal PstI site found at 310
    Illegal PstI site found at 319
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 99
    Illegal PstI site found at 310
    Illegal PstI site found at 319
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 99
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 99
    Illegal PstI site found at 310
    Illegal PstI site found at 319
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 99
    Illegal PstI site found at 310
    Illegal PstI site found at 319
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

mchI-mchB-mchX polycistrons with Ptac lacO promoter


Source

mchI-mchB-mchX polycistrons with Ptac lacO promoter

References